Recombinant:Article Title: Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research community.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CP13 Kindly provided by Peter Davies RRID:AB_2314223 AT8 Thermo Fisher Scientific Cat# MN1020; RRID:AB_223647 PHF1 Kindly provided by Peter Davies RRID:AB_2315150 TOC1 Novus Cat# NBP3-20163; RRID:AB_3608371 T22 Sigma-Aldrich Cat# ABN454; RRID:AB_2888681 MC1 Kindly provided by Peter Davies RRID:AB_2314773 Tau13 Santa Cruz Biotechnology Cat# sc-21796; RRID:AB_628328 Tau5 Thermo Fisher Scientific Cat# AHB0042; RRID:AB_2536235 MAP2 Abcum Cat# ab183830; RRID:AB_2895301 KIF5A Proteintech Cat# 21186-1-AP; RRID:AB_10733125 GA5 (Anti-GFAP) Merck Millipore Cat# MAB360; RRID:AB_11212597 Anti-Iba1 Fujifilm-wako Cat# 013–27691; RRID:AB_2934095 Synaptotagmin Synaptic System Cat# 105-002; RRID:AB_887830 Homer1 Synaptic System Cat# 160-004; RRID:AB_2619855 Synaptophysin PROGEN Cat# 61012 PSD-95 Synaptic System Cat# 124-011; RRID:AB_2619799 N1D (Anti-Ab) In-house (Saido et al. 1994) N/A Anti-b-actin, HRP Proteintech Cat# HRP-60008; RRID:AB_2819183 Anti-Rabbit IgG, HRP Proteintech Cat# SA00001-2; RRID:AB_2722564 Anti-Mouse IgG, HRP Proteintech Cat# SA00001-1; RRID:AB_2722565 Chemicals, peptides, and recombinant proteins 4% paraformaldehyde Nacalai Tesque Cat# 09154-85 RNAiso Plus Takara Bio Cat# 9109 ReverTra Ace TOYOBO Cat# FSQ-301 cOmplete protease inhibitor cocktail Roche Diagnostics Cat# 11697498001 lambda-phosphatase Santa Cruz Biotechnology Cat# sc-200312A ECL prime blocking buffer GE healthcare Cat# RPN418 ECL Select detection reagent GE Healthcare Cat# RPN2235 Tris-EDTA buffer (pH 8.0) (TE buffer) Nacalai Tesque Cat# 06890-54 0.5% blocking reagent powder AKOYA Biosciences Cat# SKU FP1012 VECTASTAIN Elite ABC-HRP Kit Vector Laboratories Cat# PK-6100 DMEM Thermo Fisher Scientific Cat# 41966-029 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985070 Herculase II Fusion DNA Polymerase Agilent Technologies Cat# 600675 mMESSAGE mMACHINE T7 Ultra Transcription kit Thermo Fisher Scientific Cat# AM1345 MEGAshortscript T7 Thermo Fisher Scientific Cat# AM1354 MEGAclear Thermo Fisher Scientific Cat# AM1908 Ex Taq-Polymerase kit Takara Bio Cat# RR001A PhosSTOP Roche Diagnostics Cat# 12352204 ProLong Gold Antifade Mountant Invitrogen Cat# P36934 (Continued on next page) e1 Cell Reports Methods 5, 101024, April 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Whole genome resequencing data (MAPTP301S; Int10 + 3; S320F) This paper SAMN46105978 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320F) This paper SAMN46105979 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320Y) This paper SAMN46105980 Experimental models: Cell lines HEK Tau RD P301S FRET biosensors ATCC Cat# CRL-3275,RRID:CVCL_DA04 Experimental models: Organisms/strains B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11768 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11769 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11770 Oligonucleotides 3R-Tau F (RT-PCR) Eurofin Genetics GTCCGTACTCCACCCAAGTC 3R-Tau R (RT-PCR) Eurofin Genetics TTTGTAGACTATTTGCACCTTCC 4R-Tau F (RT-PCR) Eurofin Genetics GAAGCTGGATCTTAGCAACG 4R-Tau R (RT-PCR) Eurofin Genetics GACGTGTTTGATATTATCCT Total Tau F (RT-PCR) Eurofin Genetics AGCCAAGACATCCACACGTT Total Tau R (RT-PCR) Eurofin Genetics ATCAGAGGGTCTGAGCTACCA G3PDH F (RT-PCR) Eurofin Genetics CCATGGCACCGTCAAGGCTGA G3PDH R (RT-PCR) Eurofin Genetics GCCAGTAGAGGCAGGGATGAT BE F Eurofin Genetics GCCGCTAATACGACTCACTATAG BE R Eurofin Genetics GCCGCTAATACGACTCACTATAG sgRNA F Eurofin Genetics TAATACGACTCACTATAGGCTCCAAG TGTGGCTCATTGTTTTAGAGCTAGAA sgRNA R Eurofin Genetics AAAAGCACCGACTCGGTGCCACTTTT TCAAGTTGATAACGGACTAGCCTTATTT TAACTTGCTATTTCTAGCTCTAAAAC Human MAPT F (common) (Genotyping) Eurofin Genetics GTCAGATCACTAGACTCAGC Human MAPT R1 (WT) (Genotyping) Eurofin Genetics CTGTGCTCCACTGTGACTGG Human MAPT R2 (MT) (Genotyping) Eurofin Genetics CTGCTTGAGTTATCTTGGCC Humanized mouse App E16WT(Genotyping) Eurofin Genetics ATCTCGGAAGTGAAGATG Humanized mouse App E16MT (Genotyping) Eurofin Genetics ATCTCGGAAGTGAATCTA Humanized mouse App WT (Genotyping) Eurofin Genetics TGTAGATGAGAACTTAAC Humanized mouse App loxP (Genotyping) Eurofin Genetics CGTATAATGTATGCTATACGAAG PS19 (Tau-Tg_P301S) F(common) (Genotyping) Eurofin Genetics TTGAAGTTGGGTTATCAATTTGG PS19 (Tau-Tg_P301S) R1 (WT) (Genotyping) Eurofin Genetics TTCTTGGAACACAAACCATTTC PS19 (Tau-Tg_P301S) R2 (MT) (Genotyping) Eurofin Genetics AAATTCCTCAGCAACTGTGGT Human MAPT_repeat identifier 3R/4R ratio F Eurofin Genetics AAGTCGCCGTCTTCCGCCAAG Human MAPT_repeat identifier 3R/4R ratio R Eurofin Genetics GTCCAGGGACCCAATCTTCGA (Continued on next page) Cell Reports Methods 5, 101024, April 21, 2025 e2 OPEN ACCESS
Protease Inhibitor:Article Title: Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research community.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CP13 Kindly provided by Peter Davies RRID:AB_2314223 AT8 Thermo Fisher Scientific Cat# MN1020; RRID:AB_223647 PHF1 Kindly provided by Peter Davies RRID:AB_2315150 TOC1 Novus Cat# NBP3-20163; RRID:AB_3608371 T22 Sigma-Aldrich Cat# ABN454; RRID:AB_2888681 MC1 Kindly provided by Peter Davies RRID:AB_2314773 Tau13 Santa Cruz Biotechnology Cat# sc-21796; RRID:AB_628328 Tau5 Thermo Fisher Scientific Cat# AHB0042; RRID:AB_2536235 MAP2 Abcum Cat# ab183830; RRID:AB_2895301 KIF5A Proteintech Cat# 21186-1-AP; RRID:AB_10733125 GA5 (Anti-GFAP) Merck Millipore Cat# MAB360; RRID:AB_11212597 Anti-Iba1 Fujifilm-wako Cat# 013–27691; RRID:AB_2934095 Synaptotagmin Synaptic System Cat# 105-002; RRID:AB_887830 Homer1 Synaptic System Cat# 160-004; RRID:AB_2619855 Synaptophysin PROGEN Cat# 61012 PSD-95 Synaptic System Cat# 124-011; RRID:AB_2619799 N1D (Anti-Ab) In-house (Saido et al. 1994) N/A Anti-b-actin, HRP Proteintech Cat# HRP-60008; RRID:AB_2819183 Anti-Rabbit IgG, HRP Proteintech Cat# SA00001-2; RRID:AB_2722564 Anti-Mouse IgG, HRP Proteintech Cat# SA00001-1; RRID:AB_2722565 Chemicals, peptides, and recombinant proteins 4% paraformaldehyde Nacalai Tesque Cat# 09154-85 RNAiso Plus Takara Bio Cat# 9109 ReverTra Ace TOYOBO Cat# FSQ-301 cOmplete protease inhibitor cocktail Roche Diagnostics Cat# 11697498001 lambda-phosphatase Santa Cruz Biotechnology Cat# sc-200312A ECL prime blocking buffer GE healthcare Cat# RPN418 ECL Select detection reagent GE Healthcare Cat# RPN2235 Tris-EDTA buffer (pH 8.0) (TE buffer) Nacalai Tesque Cat# 06890-54 0.5% blocking reagent powder AKOYA Biosciences Cat# SKU FP1012 VECTASTAIN Elite ABC-HRP Kit Vector Laboratories Cat# PK-6100 DMEM Thermo Fisher Scientific Cat# 41966-029 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985070 Herculase II Fusion DNA Polymerase Agilent Technologies Cat# 600675 mMESSAGE mMACHINE T7 Ultra Transcription kit Thermo Fisher Scientific Cat# AM1345 MEGAshortscript T7 Thermo Fisher Scientific Cat# AM1354 MEGAclear Thermo Fisher Scientific Cat# AM1908 Ex Taq-Polymerase kit Takara Bio Cat# RR001A PhosSTOP Roche Diagnostics Cat# 12352204 ProLong Gold Antifade Mountant Invitrogen Cat# P36934 (Continued on next page) e1 Cell Reports Methods 5, 101024, April 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Whole genome resequencing data (MAPTP301S; Int10 + 3; S320F) This paper SAMN46105978 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320F) This paper SAMN46105979 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320Y) This paper SAMN46105980 Experimental models: Cell lines HEK Tau RD P301S FRET biosensors ATCC Cat# CRL-3275,RRID:CVCL_DA04 Experimental models: Organisms/strains B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11768 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11769 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11770 Oligonucleotides 3R-Tau F (RT-PCR) Eurofin Genetics GTCCGTACTCCACCCAAGTC 3R-Tau R (RT-PCR) Eurofin Genetics TTTGTAGACTATTTGCACCTTCC 4R-Tau F (RT-PCR) Eurofin Genetics GAAGCTGGATCTTAGCAACG 4R-Tau R (RT-PCR) Eurofin Genetics GACGTGTTTGATATTATCCT Total Tau F (RT-PCR) Eurofin Genetics AGCCAAGACATCCACACGTT Total Tau R (RT-PCR) Eurofin Genetics ATCAGAGGGTCTGAGCTACCA G3PDH F (RT-PCR) Eurofin Genetics CCATGGCACCGTCAAGGCTGA G3PDH R (RT-PCR) Eurofin Genetics GCCAGTAGAGGCAGGGATGAT BE F Eurofin Genetics GCCGCTAATACGACTCACTATAG BE R Eurofin Genetics GCCGCTAATACGACTCACTATAG sgRNA F Eurofin Genetics TAATACGACTCACTATAGGCTCCAAG TGTGGCTCATTGTTTTAGAGCTAGAA sgRNA R Eurofin Genetics AAAAGCACCGACTCGGTGCCACTTTT TCAAGTTGATAACGGACTAGCCTTATTT TAACTTGCTATTTCTAGCTCTAAAAC Human MAPT F (common) (Genotyping) Eurofin Genetics GTCAGATCACTAGACTCAGC Human MAPT R1 (WT) (Genotyping) Eurofin Genetics CTGTGCTCCACTGTGACTGG Human MAPT R2 (MT) (Genotyping) Eurofin Genetics CTGCTTGAGTTATCTTGGCC Humanized mouse App E16WT(Genotyping) Eurofin Genetics ATCTCGGAAGTGAAGATG Humanized mouse App E16MT (Genotyping) Eurofin Genetics ATCTCGGAAGTGAATCTA Humanized mouse App WT (Genotyping) Eurofin Genetics TGTAGATGAGAACTTAAC Humanized mouse App loxP (Genotyping) Eurofin Genetics CGTATAATGTATGCTATACGAAG PS19 (Tau-Tg_P301S) F(common) (Genotyping) Eurofin Genetics TTGAAGTTGGGTTATCAATTTGG PS19 (Tau-Tg_P301S) R1 (WT) (Genotyping) Eurofin Genetics TTCTTGGAACACAAACCATTTC PS19 (Tau-Tg_P301S) R2 (MT) (Genotyping) Eurofin Genetics AAATTCCTCAGCAACTGTGGT Human MAPT_repeat identifier 3R/4R ratio F Eurofin Genetics AAGTCGCCGTCTTCCGCCAAG Human MAPT_repeat identifier 3R/4R ratio R Eurofin Genetics GTCCAGGGACCCAATCTTCGA (Continued on next page) Cell Reports Methods 5, 101024, April 21, 2025 e2 OPEN ACCESS
Blocking Assay:Article Title: Human MAPT knockin mouse models of frontotemporal dementia for the neurodegenerative research community.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies CP13 Kindly provided by Peter Davies RRID:AB_2314223 AT8 Thermo Fisher Scientific Cat# MN1020; RRID:AB_223647 PHF1 Kindly provided by Peter Davies RRID:AB_2315150 TOC1 Novus Cat# NBP3-20163; RRID:AB_3608371 T22 Sigma-Aldrich Cat# ABN454; RRID:AB_2888681 MC1 Kindly provided by Peter Davies RRID:AB_2314773 Tau13 Santa Cruz Biotechnology Cat# sc-21796; RRID:AB_628328 Tau5 Thermo Fisher Scientific Cat# AHB0042; RRID:AB_2536235 MAP2 Abcum Cat# ab183830; RRID:AB_2895301 KIF5A Proteintech Cat# 21186-1-AP; RRID:AB_10733125 GA5 (Anti-GFAP) Merck Millipore Cat# MAB360; RRID:AB_11212597 Anti-Iba1 Fujifilm-wako Cat# 013–27691; RRID:AB_2934095 Synaptotagmin Synaptic System Cat# 105-002; RRID:AB_887830 Homer1 Synaptic System Cat# 160-004; RRID:AB_2619855 Synaptophysin PROGEN Cat# 61012 PSD-95 Synaptic System Cat# 124-011; RRID:AB_2619799 N1D (Anti-Ab) In-house (Saido et al. 1994) N/A Anti-b-actin, HRP Proteintech Cat# HRP-60008; RRID:AB_2819183 Anti-Rabbit IgG, HRP Proteintech Cat# SA00001-2; RRID:AB_2722564 Anti-Mouse IgG, HRP Proteintech Cat# SA00001-1; RRID:AB_2722565 Chemicals, peptides, and recombinant proteins 4% paraformaldehyde Nacalai Tesque Cat# 09154-85 RNAiso Plus Takara Bio Cat# 9109 ReverTra Ace TOYOBO Cat# FSQ-301 cOmplete protease inhibitor cocktail Roche Diagnostics Cat# 11697498001 lambda-phosphatase Santa Cruz Biotechnology Cat# sc-200312A ECL prime blocking buffer GE healthcare Cat# RPN418 ECL Select detection reagent GE Healthcare Cat# RPN2235 Tris-EDTA buffer (pH 8.0) (TE buffer) Nacalai Tesque Cat# 06890-54 0.5% blocking reagent powder AKOYA Biosciences Cat# SKU FP1012 VECTASTAIN Elite ABC-HRP Kit Vector Laboratories Cat# PK-6100 DMEM Thermo Fisher Scientific Cat# 41966-029 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985070 Herculase II Fusion DNA Polymerase Agilent Technologies Cat# 600675 mMESSAGE mMACHINE T7 Ultra Transcription kit Thermo Fisher Scientific Cat# AM1345 MEGAshortscript T7 Thermo Fisher Scientific Cat# AM1354 MEGAclear Thermo Fisher Scientific Cat# AM1908 Ex Taq-Polymerase kit Takara Bio Cat# RR001A PhosSTOP Roche Diagnostics Cat# 12352204 ProLong Gold Antifade Mountant Invitrogen Cat# P36934 (Continued on next page) e1 Cell Reports Methods 5, 101024, April 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Whole genome resequencing data (MAPTP301S; Int10 + 3; S320F) This paper SAMN46105978 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320F) This paper SAMN46105979 Whole genome resequencing data (MAPTS305N; Int10 + 3; S320Y) This paper SAMN46105980 Experimental models: Cell lines HEK Tau RD P301S FRET biosensors ATCC Cat# CRL-3275,RRID:CVCL_DA04 Experimental models: Organisms/strains B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11768 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11769 B6.Cg-MaptA)Tcs> RIKEN BRC RBRC11770 Oligonucleotides 3R-Tau F (RT-PCR) Eurofin Genetics GTCCGTACTCCACCCAAGTC 3R-Tau R (RT-PCR) Eurofin Genetics TTTGTAGACTATTTGCACCTTCC 4R-Tau F (RT-PCR) Eurofin Genetics GAAGCTGGATCTTAGCAACG 4R-Tau R (RT-PCR) Eurofin Genetics GACGTGTTTGATATTATCCT Total Tau F (RT-PCR) Eurofin Genetics AGCCAAGACATCCACACGTT Total Tau R (RT-PCR) Eurofin Genetics ATCAGAGGGTCTGAGCTACCA G3PDH F (RT-PCR) Eurofin Genetics CCATGGCACCGTCAAGGCTGA G3PDH R (RT-PCR) Eurofin Genetics GCCAGTAGAGGCAGGGATGAT BE F Eurofin Genetics GCCGCTAATACGACTCACTATAG BE R Eurofin Genetics GCCGCTAATACGACTCACTATAG sgRNA F Eurofin Genetics TAATACGACTCACTATAGGCTCCAAG TGTGGCTCATTGTTTTAGAGCTAGAA sgRNA R Eurofin Genetics AAAAGCACCGACTCGGTGCCACTTTT TCAAGTTGATAACGGACTAGCCTTATTT TAACTTGCTATTTCTAGCTCTAAAAC Human MAPT F (common) (Genotyping) Eurofin Genetics GTCAGATCACTAGACTCAGC Human MAPT R1 (WT) (Genotyping) Eurofin Genetics CTGTGCTCCACTGTGACTGG Human MAPT R2 (MT) (Genotyping) Eurofin Genetics CTGCTTGAGTTATCTTGGCC Humanized mouse App E16WT(Genotyping) Eurofin Genetics ATCTCGGAAGTGAAGATG Humanized mouse App E16MT (Genotyping) Eurofin Genetics ATCTCGGAAGTGAATCTA Humanized mouse App WT (Genotyping) Eurofin Genetics TGTAGATGAGAACTTAAC Humanized mouse App loxP (Genotyping) Eurofin Genetics CGTATAATGTATGCTATACGAAG PS19 (Tau-Tg_P301S) F(common) (Genotyping) Eurofin Genetics TTGAAGTTGGGTTATCAATTTGG PS19 (Tau-Tg_P301S) R1 (WT) (Genotyping) Eurofin Genetics TTCTTGGAACACAAACCATTTC PS19 (Tau-Tg_P301S) R2 (MT) (Genotyping) Eurofin Genetics AAATTCCTCAGCAACTGTGGT Human MAPT_repeat identifier 3R/4R ratio F Eurofin Genetics AAGTCGCCGTCTTCCGCCAAG Human MAPT_repeat identifier 3R/4R ratio R Eurofin Genetics GTCCAGGGACCCAATCTTCGA (Continued on next page) Cell Reports Methods 5, 101024, April 21, 2025 e2 OPEN ACCESS
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